Tsingke tse005 2×t5 super pcr mix colony
WebJul 18, 2024 · Polymerase Chain Reaction (PCR) was performed in 12 μl reaction volumes containing 2 template DNA, 5 μl of 2 × T5 Super PCR Mix (PAGE; TSINGKE, Beijing, China), 1 μl of 10 μmol/μL primer pairs, and 4 μl of double distilled H 2 O. Target DNA segments were amplified with the following program, 95°C for 5 min, followed by 35 cycles of 95°C for 10 … WebSep 8, 2015 · PrimeSTAR Max polymerase (2× Master Mix, Takara Bio) was used to generate both altered plasmids. PCR reactions were digested with DpnI for ~2 hours at 37°C and purified with Nucleospin Gel and PCR Clean-Up kit (Macherey-Nagel). 1 μl of the purified product (125–150 ng DNA) was combined with 25 μl NEB 5-alpha chemically competent …
Tsingke tse005 2×t5 super pcr mix colony
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WebJun 9, 2024 · Tsingke Biotech leads the way in the development & manufacturing of genomic and biosynthesis products for global researchers. ... 2×T5 Supper PCR … Web丁香通为您找到202条pcr一侧产物出现套峰厂家的工商注册年份、员工人数、年营业额、信用记录、主营产品、相关pcr一侧产物出现套峰产品的供求信息、交易记录等企业详情。丁香通为买家提供用户服务，诚信保障等服务，批发采购pcr一侧产物出现套峰，上丁香通。
WebqRT-PCR Analysis For qRT-PCR analysis, we extracted RNA using the RNAprep Pure Plant Kit (TIANGEN, Beijing, ... The 2 × T5 Super PCR Mix (Colony) was used. ... WebNov 20, 2024 · Abstract. The assembly of DNA fragments with homologous arms is becoming popular in routine cloning. For an in vitro assembly reaction, a DNA polymerase is often used either alone for its 3′-5′ exonuclease activity or together with a 5′-3′ exonuclease for its DNA polymerase activity. Here, we present a ‘T5 exonuclease DNA assembly’ …
WebMay 25, 2024 · We use a homemade 2× PCR master mix containing DNA polymerase, buffer, Mg 2+, dNTPs, and DMSO (see Subheading 2) to which are added PCR primers to a final concentration of 1 μM and water. We prepare 110% of the theoretical required volume, which is calculated as the total volume of each PCR reaction times the number of clones, … WebMoreover, compared to a commercially available kit (2 × T5 Direct PCR kit) (Tsingke, Beijing, China), the electrophoresis results of this colony PCR was also satisfactory with specific …
Web我们联系：[email protected]。 2×T5 Super PCR Mix (PAGE) TSE006 产品组成： PCR体系与条件： 目录号： 2×T5 Super PCR Mix (PAGE) 5×1.0 ml Component 2×T5 Super PCR Mix (PAGE) 10 μM Forward Primer 10 μM Reverse Primer Template DNA Nuclease-Free Water Final Concentration 1× 0.4 μM 0.4 μM <1 μg 推荐反应体系 ...
WebCitations & References. PCR SuperMix is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification. Simply add template and primers, … portsmouth new hampshire mazdaWeb2×T5 Super PCR Mix (Colony) TSE005 产品说明： 目录号： T5 DNA 聚合酶是新型DNA 聚合酶，由经过改造的Taq DNA 聚合酶融合Sso7d DNA结合域而得来，具有极高的扩增 速度 … portsmouth new englandWeb2 ×T5 Super PCR Mix (Colony) 5. Initial denaturation. 98. 30s. 1 × . Forward primer . 0.2. Denaturation. 98. 10s. 29 × . Feverse primer . 0.2. Annealing. T m-5. 30s. Template. 2. … portsmouth new hampshire new years eveWebA 2× T5 Super PCR Mix (Colony) (cat. no. TSE005, Tsingke, Beijing, China) was used to identify the genes of single bacteria. Amplifications were performed in a 25 μL reaction … or condition in if in xsltWebMCLAB Products. I-5™ 2X Hi-Fi PCR Master Mix. I-5 High-Fidelity DNA Polymerase is an ultra-high fidelity and high processivity enzyme. It produces the most accurate copies of … portsmouth new hampshire passport officeWebFeb 18, 2024 · Prime STAR Max DNA polymerase was bought from Takara (Shanghai, China), T5 super PCR Mix (Colony) DNA polymerase, ... Trelidf™ Prestained Protein … portsmouth new hampshire radar mapWebApr 2, 2024 · GoldView TM Type I Nucleic Acid Electrophoresis Dye was purchased from Solarbio (Beijing, China), and 2×T5 Super PCR Mix was bought from TSINGKE (Hangzhou, … or condition in if batch script