The blue–white screen is a screening technique that allows for the rapid and convenient detection of recombinant bacteria in vector-based molecular cloning experiments. This method of screening is usually performed using a suitable bacterial strain, but other organisms such as yeast may also be used. DNA of transformation is ligated into a vector. The vector is then inserted into a compet… WebProduct Description. Bluo-Gal is used as an alternative to X-Gal, and produces a darker blue color. Bluo-Gal is hydrolyzed by β-galactosidase to produce a blue precipitate. This precipitate is insoluble in alcohol and xylenes, thus making it suitable for immunoblotting and immunocytochemical assays. Bacterial colonies containing active β ...
Competent Cells Support – Getting Started - Thermo Fisher Scientific
WebPreparation of X-Gal/IPTG LB Agar Plates for Blue/White Colony Screening For individual LB (Luria Broth) agar plates: 1. Pour sterile warm LB agar (about 25 mL) into a Petri dish. 2. … WebJul 14, 2016 · In blue white screening of recombinant and non recombinants using IPTG-Xgal I got colour series of blue colonies. Some are very dark and some are pale in color intensity. And I can agree... share your photo bilder löschen
Bluo-Gal GoldBio
WebApr 15, 2024 · Blue/white visual screening of the recombinants is enabled by inserting the target genes into the lacZ α-peptide coding region in an antisense ... As with the standard pET vectors, the target proteins are produced by IPTG induction. The following components were oriented to prepare PCR products for cloning: blunt vector (50 ng/µl), positive ... WebFeb 16, 2024 · A new partnership aims to improve the health of Black metro Detroiters by offering free screenings, treatment and medicine to target undiagnosed and uncontrolled … WebBlue-white screening of bacterial colonies using IPTG and X-Gal. Materials X-Gal (GoldBio Catalog # X4281C ) Dimethylformamide (DMF) dH 2O Isopropyl β-D-1 … share your photo de