Web10 de abr. de 2024 · Pdgfra is a robust marker for dermal fibroblasts and is expressed at ... with oligo(dT) primers. qPCR was conducted with the Hieff qPCR SYBR Green Master Mix (Yeasen Biotech, cat: 11202ES03 ... Web29 de abr. de 2024 · The amplification mix contained 2 × Hieff ® Robust PCR Master Mix (dNTP and Mg 2+) (Yeasen Co. Ltd., Shanghai, China), 1 μl of each primer, 10–20 ng of PCR products, and 9–12 μl H 2 O in a reaction volume of 30 μl.
2×Hieff ® Robust PCR Master Mix(With Dye)
Web6 de dez. de 2024 · Polymerase chain reaction (PCR) was performed with 2×Hieff® Robust PCR Master Mix (YEASEN, China) and the products were detected by agarose gel electrophoresis. Web30 de jun. de 2024 · The PCR assay volume was 30 μL and included 15 μL 2 × Hieff® Robust PCR Master Mix, 1 μL of the 10 μm forward and reverse primers, and 10–20 g of DNA template; ddH 2 O was added to 30 μL. eagles coach crys
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Web研发背景. Hieff ® Robust PCR Master Mix是一款高检出率型PCR预混液,通过酶结构的改造、筛选及Buffer的优化,解决了常规PCR Mix检出率低、产量低的问题。 另外,延伸速度提升至15-30 s/kb,可用于基因的快速克隆。 产品描述. Hieff ® Robust PCR Master Mix是预混PCR反应所需的各类组分的预混液,仅需加入模板和引 ... Web1 de out. de 2024 · In order to investigate the role of ALDH2C4 on cuticle, two mutants of ALDH2C4 were identified by molecular means. Two ALDH2C4 mutant lines, aldh2c4–1 (Salk_027911C) and aldh2c4–2 (Salk_024974C), which carry homozygous T-DNA insertions, were identified by PCR analysis (Fig. S2).To confirm the position of the T … Web安毅夫,孙 娟,高 扬,连国玺,杨 冰 (中核第四研究设计工程有限公司,河北 石家庄 050021) 铀尾渣作为铀矿采冶产生的主要固体废物,其不仅含有U、226Ra、210Pb、210Po 等天然放射性核素,也包括水冶加工工艺带入的酸、碱、有机物等多种化工物料,在降水淋滤和地表径流的作用下[1],导致污染物的溶出和迁移 ... eagles coaches 2022